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Troponin certain to heparin triggers decrease calculated troponin plasma focus compared to serum.[seventy six] Other sources of interference that may have an affect on the assay detection course of action and result in Untrue-negative troponin values incorporate ascorbic acid in immunoenzymometric assays making use of alkaline phosphatase, biotin in assays employing a biotinylated antibody, streptokinase inside the existence of streptavidin, and superior titers of antibodies to ruthenium or streptavidin in cTnT assays.
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The captured troponin is then reacted by using a next antibody and, in a few assays, a 3rd antibody coupled to an indicator molecule.[36] The assays vary from one another by the categories of antibody used, because of the epitopes to which they bind, and by the type of indicator molecule that may be utilised.[46]
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